Inhalation Route Research Articles

Microfluidic Platform Enables Shearless Aerosolization of Lipid Nanoparticles for mRNA Inhalation.

Leveraging the extensive surface area of the lungs for gene therapy, the inhalation route offers distinct advantages for delivery. Clinical nebulizers that employ vibrating mesh technology are the standard choice for converting liquid medicines into aerosols. However, they have limitations when it comes to delivering mRNA through inhalation, including severe damage to nanoparticles due to shearing forces. Here, we introduce a microfluidic aerosolization platform (MAP) that preserves the structural and physicochemical integrity of lipid nanoparticles, enabling safe and efficient delivery of mRNA to the respiratory system. Our results demonstrated the superiority of the MAP over the conventional vibrating mesh nebulizer, as it avoided problems such as particle aggregation, loss of mRNA encapsulation, and deformation of the nanoparticle morphology. Notably, aerosolized nanoparticles generated by the microfluidic device led to enhanced transfection efficiency across various cell lines. In vivo experiments with mice that inhaled these aerosolized nanoparticles revealed successful lung-specific mRNA transfection without observable signs of toxicity. This MAP may represent an advancement for the pulmonary gene therapy, enabling precise and effective delivery of aerosolized nanoparticles.

Comparative evaluation of nebulized versus intravenous dexmedetomidine on intubating conditions during awake fiberoptic nasotracheal intubation

BackgroundThere is a search for an ideal agent to facilitate awake fiberoptic intubation (AFOI). Dexmedetomidine is a selective α2 agonist which can be administered through intravenous, intramuscular, buccal, intranasal & inhalational routes. It provides good intubation conditions without oxygen desaturation but may cause hypotension and bradycardia when administered intravenously. Hence, alternative routes of administering dexmedetomidine which may improve its safety profile are worth exploring. MethodsIn this randomised, controlled, double-blind trial, 46 ASA I/II adult participants scheduled for elective ENT surgery were randomly allocated to Group ND (Nebulised Dexmedetomidine) (n = 23) to receive nebulisation with dexmedetomidine 1μg.kg−1 and Group ID (Intravenous Dexmedetomidine) (n = 23) to receive intravenous dexmedetomidine 1μg.kg−1 before AFOI. All the patients received injection midazolam 1 mg i.v. as premedication before anaesthesia was initiated. The primary outcome was the cough score. The secondary outcomes were the RSS, SAYGO boluses, post-intubation score, hemodynamic parameters, recall of the procedure, patient satisfaction score and any side effects. ResultsThe cough score was significantly lower in nebulized group (2.43 ± 0.992 vs 3.52 ± 1.082) with p = 0.001. RSS(3.30 ± 0.926 vs 4.22 ± 1.126; p = 0.004), number of SAYGO boluses required (2.74 ± 0.864 vs 3.57 ± 1.161; p = 0.009) & the post intubation score (1.48 ± 0.593 vs 2.17 ± 0.778; p = 0.001) were also significantly lower in nebulized group. ConclusionsNebulisation with dexmedetomidine results in desirable degree of sedation and better tolerance of the procedure with adequate attenuation of the haemodynamic responses to intubation.

Effect of Hemerocallis essence aroma on brain activity and sleep processes via scalp electroencephalogram

AbstractSleep disorders are widespread. Inhaled aromatherapy has shown advantages over medication in treating insomnia. Daylily (Hemerocallis fulva) has been found to positively affect sleep either by ingestion or inhalation, but there is a lack of research on the sleep aid effect of its inhalation route. At present, the floral scent characteristics of daylily are not obvious, and the extraction rate of essential oil is low. Based on the above problems, this study developed daylily essence which replicates the natural aroma of plants and investigated the effect of inhaling daylily essence aroma on sleep activity by using Pittsburgh Sleep Quality Index (PSQI) and electroencephalogram (EEG). According to PSQI results, inhaling the aroma of daylily essence improved participants' subjective sleep quality. The difference between men and women was statistically significant (p < .05). The slow‐wave sleep activity of EEG in the experimental group was significantly stronger than that in the control group (p < .01). In addition, the frontal and central regions were more responsive to aroma stimuli (p < .05). The results of this study show that the aroma of daylily can promote the subjective and objective sleep quality of healthy college students, and the use of fragrance intervention is a potential scheme to improve sleep quality and insomnia.

Sonicated polyethylene terephthalate nano- and micro-plastic-induced inflammation, oxidative stress, and autophagy in vitro

The environmental presence of nano- and micro-plastic particles (NMPs) is suspected to have a negative impact on human health. Environmental NMPs are difficult to sample and use in life science research, while commercially available plastic particles are too morphologically uniform. Additionally, this NMPs exposure exhibited biological effects, including cell internalization, oxidative stress, inflammation, cellular adaptation, and genotoxicity. Therefore, developing new methods for producing heterogenous NMPs as observed in the environment is important as reference materials for research. Thus, we aimed to generate and characterize NMPs suspensions using a modified ultrasonic protocol and to investigate their biological effects after exposure to different human cell lines. To this end, we produced polyethylene terephthalate (PET) NMPs suspensions and characterized the particles by dynamic light scattering and scanning electron microscopy. Ultrasound treatment induced polymer degradation into smaller and heterogeneous PET NMPs shape fragments with similar surface chemistry before and after treatment. A polydisperse suspension of PET NMPs with 781 nm in average size and negative surface charge was generated. Then, the PET NMPs were cultured with two human cell lines, A549 (lung) and HaCaT (skin), addressing inhalation and topical exposure routes. Both cell lines interacted with and have taken up PET NMPs as quantified via cellular granularity assay. A549 but not HaCaT cell metabolism, viability, and cell death were affected by PET NMPs. In HaCaT keratinocytes, large PET NMPs provoked genotoxic effects. In both cell lines, PET NMPs exposure affected oxidative stress, cytokine release, and cell morphology, independently of concentration, which we could relate mechanistically to Nrf2 and autophagy activation. Collectively, we present a new PET NMP generation model suitable for studying the environmental and biological consequences of exposure to this polymer.

Oznaczanie 1,2-dihydroksybenzenu w powietrzu na stanowiskach pracy metodą chromatografii gazowej z detekcją płomieniowo-jonizacyjną

1,2-Dihydroxybenzene is a colorless crystalline substance with a characteristic odor that turns brown when exposed to air and light. It is used in industry as an antioxidant. Worker exposure to 1,2-dihydroxybenzene can occur during the production, processing and use of the chemical, through inhalation, dermal and gastrointestinal routes. The aim of the study was to develop a method for the determination of 1,2-dihydroxybenzene to assess occupational exposure within 1/10–2 of the proposed MAC value (10 mg/m3 ). The method involves the collection of 1,2-dihydroxybenzene in a system consisting of a glass fiber filter and a tube containing two layers of XAD-7 sorbent, extraction with a solution of N,N-dimethylformamide in methanol, and chromatographic analysis of the resulting solution. The method allows the determination of 1,2-dihydroxybenzene in air in the concentration range from 1.0 to 20.0 mg/m3 . The method has been validated in accordance with PN-EN 482. The scope of the article includes health and environmental health and safety issues being the subject of research in health sciences and environmental engineering.

Internalization and toxicity of polystyrene nanoplastics on inmortalized human neural stem cells

Global plastic production has increased exponentially in recent decades, and a significant part of it persists in the environment, where it degrades into microplastics and nanoplastics (MPs and NPs). These can enter in humans by ingestion, inhalation, and dermal routes, and there is scientific evidence that they are able to reach the systemic circulation and penetrate and accumulate in various tissues and organs. Neurodevelopmental toxicity of NPs is one of the most worrying effects, as they can cross the blood-brain barrier. In the following study, we analyzed, by transmission electron microscopy, the in vitro uptake of 30-nm polystyrene nanoplastics (PS-NPs) into human neural stem cells (NSCs), their accumulation and subcellular localization within the cell. Furthermore, we studied the effects of different concentrations of PS-NPs on cell death, proliferation, and cell differentiation using immunocytochemistry and quantitative real time PCR for specific markers. This study demonstrated that PS-NPs were able to enter the cell, probably by endocytosis, accumulate, and aggregated in human NSCs, without being detected in the nucleus, causing cell death by apoptosis and decreased cell proliferation. This study provides new insights into the interaction and effects of PS-NPs in human NSC and supports the scientific evidence for the involvement of nanoplastic in neurodevelopmental disorders.

Human Health Effects of Oral Exposure to Chromium: A Systematic Review of the Epidemiological Evidence

The toxicity and carcinogenicity of hexavalent chromium via the inhalation route is well established. However, a scientific debate has arisen about the potential effects of oral exposure to chromium on human health. Epidemiological studies evaluating the connection between ingested chromium and adverse health effects on the general population are limited. In recent years, a wealth of biomonitoring studies has emerged evaluating the associations between chromium levels in body fluids and tissues and health outcomes. This systematic review brings together epidemiological and biomonitoring evidence published over the past decade on the health effects of the general population related to oral exposure to chromium. In total, 65 studies were reviewed. There appears to be an inverse association between prenatal chromium exposure and normal fetal development. In adults, parameters of oxidative stress and biochemical alterations increase in response to chromium exposure, while effects on normal renal function are conflicting. Risks of urothelial carcinomas cannot be overlooked. However, findings regarding internal chromium concentrations and abnormalities in various tissues and systems are, in most cases, controversial. Environmental monitoring together with large cohort studies and biomonitoring with multiple biomarkers could fill the scientific gap.

Effect of Nasal Inhalation on Drug Particle Deposition and Size Distribution in the Upper Airway: With Soft Mist Inhalers.

Delivery of drugs to the lungs is commonly achieved using nasal and/or oral breathing-assisted techniques. The route of inhalation can substantially change the fate of inhaled droplets. The Respimat® Soft Mist™ Inhaler (SMI) is a commercially available efficient inhaler with 40-60% effectiveness. In the present study, we used computational fluid dynamics (CFD) with a custom setup to investigate the effect of a combined oral/nasal inhalation route on the SMI's regional droplet deposition, size distribution, and flow field. Our setup used a modified induction port (MIP) to mimic nasal inhalation inside the human respiratory tract. Six different oral/nasal flow rate ratios inside the MIP were applied (total flow rate of 30l/min). An overall good agreement was achieved between simulation outcomes and in vitro results. Our results confirmed that the combined inhalation route affects the flow field, altering the MIP's droplet deposition and size distribution. The lowest depositional loss, mainly in the mouth area, was observed at oral/nasal flow rate ratios of O/N = 1 and O/N = 2 with 3% and 7.7% values, respectively. Droplets with a 2-5µm diameter range showed the highest droplet mass inside the MIP at all combined flow rates. We observed less intense vortexes followed by a lower level of turbulent kinetic energy at the oral/nasal ratio of 1. Increasing the relative humidity (RH) at oral/nasal flow rate ratios of 0.07, 1, and 14 led to an increase in droplet deposition at the outlet of the MIP.

Pharmacotherapeutic Considerations in the Treatment of Nontuberculous Mycobacterial Infections: A Primer for Clinicians.

Nontuberculous mycobacteria (NTM) can cause a variety of infections, including serious pulmonary disease. Treatment encompasses polypharmacy, with a targeted regimen of 2-5 active medications, depending on site of infection, species, and clinical characteristics. Medications may include oral, intravenous, and inhalational routes. Medication acquisition can be challenging for numerous reasons, including investigational status, limited distribution models, and insurance prior authorization. Additionally, monitoring and managing adverse reactions and drug interactions is a unique skill set. While NTM is primarily medically managed, clinicians may not be familiar with the intricacies of medication selection, procurement, and monitoring. This review offers insights into the pharmacotherapeutic considerations of this highly complex disease state, including regimen design, medication acquisition, safety monitoring, relevant drug-drug interactions, and adverse drug reactions.

Assessment of nanocarrier mediated afatinib pharmacokinetic: A comparative in-vivo study in rat model for enhanced therapeutic efficacy in lung cancer management

Nanocarrier-based drug delivery systems are at the forefront of innovative research in the field of medical science, providing potential pathways for treatments across several routes of administration. Notably, a great deal of research has gone into maximizing the potential of polymeric nanoparticles and liposomes, with a focus on addressing the intricacies of lung cancer through the pulmonary route. The present research aims to improve the pharmacokinetic profile of afatinib using a liposome and polymeric nanoparticle based dry powder approach rather than the afatinib plain drug solution to increase the survival rate and improvise the treatment of patients suffering from lung cancer. Formulated afatinib encapsulated PEGylated liposomal and afatinib loaded PLGA nanoparticles dry powder inhaler were characterized for the in-vivo pulmonary pharmacokinetics study using rat model upon intratracheal administration. The results of in-vivo pulmonary pharmacokinetics studies of both formulations indicated that it improved the pharmacokinetics profiles of the encapsulated agent (afatinib) by increasing the Cmax, AUC Total, Mean residence time (MRT) and decreasing the clearance (CL), and volume of distribution (VZ) than the afatinib plain drug solution. Results suggested that the selective targeting ability of the nanocarrier system can be a favourable approach for the management of lung cancer by the inhalation route.

State-of-the-Art Review on Inhalable Lipid and Polymer Nanocarriers: Design and Development Perspectives.

Nowadays, the interest in research towards the local administration of drugs via the inhalation route is growing as it enables the direct targeting of the lung tissue, at the same time reducing systemic side effects. This is of great significance in the era of nucleic acid therapeutics and personalized medicine for the local treatment of severe lung diseases. However, the success of any inhalation therapy is driven by a delicate interplay of factors, such as the physiochemical profile of the payload, formulation, inhalation device, aerodynamic properties, and interaction with the lung fluids. The development of drug delivery systems tailored to the needs of this administration route is central to its success and to revolutionize the treatment of respiratory diseases. With this review, we aim to provide an up-to-date overview of advances in the development of nanoparticulate carriers for drug delivery to the lung tissue, with special regard concerning lipid and polymer-based nanocarriers (NCs). Starting from the biological barriers that the anatomical structure of the lung imposes, and that need to be overcome, the current strategies to achieve efficient lung delivery and the best support for the success of NCs for inhalation are highlighted.

CeO2-based peelable gel for neutralization and skin decontamination toward chemical warfare agents

Accidents with toxic industrial chemicals and threats posed by chemical warfare agents, as organophosphorus nerve agents and blistering agents, has become an increasing concern over the last decades. Intoxication is not only due to inhalation but dermal route can also be important and can quickly lead to death. It is therefore crucial to have suitable means of personal protection and decontamination. Some are already commercially available but often present drawbacks. Efforts have been made to develop new systems and several studies have shown the interest of metallic nanoparticles. The challenge remains to entrap the particles in an adapted matrix to limit their diffusion in the immediate environment, while preserving the bare particles decontamination efficacy. In this study, an easy to handle peelable gel, incorporating CeO2 nanoparticles has been developed for efficient skin decontamination against toxic chemicals. The main objective has been to make it suitable in any context of application (battlefield or civil exposure). Nano-octahedra (NO) CeO2 particles synthetized in this study have been able to increase the degradation of paraoxon, a simulant warfare agent in liquid media. NO have then been incorporated in a peelable gel, the formulation has been optimized through design of experiments and its physico-chemical properties characterized. Even after incorporation to the gel, NO retained their neutralization properties. The formulation efficiency towards neutralization and skin decontamination have been evaluated in vitro on Franz diffusion cells. The interest of the peelable gel have been clearly demonstrated with a decrease by about 5-fold of the quantity of POX absorbed into the skin and a decrease of 3–4-fold in the viable skin, which was much higher than the use of cotton or nanoparticles as powder.

Forensic investigation on a combined death by food aspiration and acute escitalopram intoxication occurred to a psychiatric subject in a nursing home.

Food aspiration is one of the major health risks for elderly people in nursing homes which could lead to death. Moreover, misconducts in pharmacotherapy may represent a potential risk of adverse drug reactions. It is reported here the toxicological evaluation of a combined death by food aspiration and acute escitalopram intoxication of a psychiatric subject, occurred in a nursing home. An 89-year-old man, suffering from dysphagia and Alzheimer's, was resident in a nursing home. He was fed with a liquid diet administered directly in mouth using a syringe. The man was also being treated with escitalopram 10mg tablet. One evening, after receiving the meal in the usual way, the man complained of sudden illness. Carried to the emergency room, the man died about 3h later with a diagnosis of cardiogenic shock subsequentially to ab ingestis. The histological findings revealed the presence of exogenous material, probably food, up to the finest bronchial branches. The toxicological examination revealed the presence of escitalopram and its main metabolite, desmethylcitalopram: in the blood 1972ng/ml and 285ng/ml, in the brain 4657ng/g and 1025ng/g, in the gastric content 2317ng/g and 423ng/g, in the lung 21,771ng/g and 468ng/g, respectively. The bad practice of the nurses to dissolve the escitalopram tablet in the liquefied food and to administer the therapy with a syringe directly into the mouth emerged thanks this investigation. Following food aspiration, escitalopram was absorbed by inhalation route, reaching high concentrations in blood and tissues. The death occurred due to a combined mechanism between food aspiration and the escitalopram toxic action.

A Study of Clinical and Socio-Demographic Profile of Elderly Patients with Opioid Use Disorder on Agonist Treatment Attending a Tertiary Care Teaching Institute of India

Background: Opioid use is one among the major substance of use in Indian population which is not only seen in younger age group but rather is an upcoming concern in the elderly population as well. Opioid agonist treatment for opioid use disorder is a better way of management, among which buprenorphine is a safer option. Despite, being an important area of health concern, opioid use is poorly studied in the geriatric population. Objective: To study the clinical and socio-demographic profile of elderly patients with opioid use disorder on agonist treatment attending a tertiary care teaching institute of urban Delhi. Method: A retrospective study was done in an OPD based opioid treatment centre of a tertiary care hospital in Delhi to analyze the socio-demographic, clinical profile, pattern of opioid use disorder and opioid agonist treatment (Buprenorphine) in elderly Indian population over a year. The data collected was further analyzed for descriptive and analytic statistics using SPSS version 23.0. Results: A total of 32 subjects of age > 60years, with predominant male population having mean age of 63.6years we seen. They had heroin use by inhalation route as a major opioid use. The main source of referral was by friends and self with significant medical and psychiatric comorbidities that was seen in the elderly group. An average dose of 2.34mg of Buprenorphine was found to effectively manage the geriatric opioid use disorder. Conclusion: Opioid Used Disorder is common in Geriatric population and is often associated with both psychiatric and medical co-morbidities. Opioid agonist treatment is an effective approach in management of elderly cases of opioid use disorder. Declaration of Interest : None

Citrus clementine Peel Essential Oil Ameliorates Potassium Dichromate-Induced Lung Injury: Insights into the PI3K/AKT Pathway.

Acute Lung Injury (ALI) is a life-threatening syndrome that has been identified as a potential complication of COVID-19. There is a critical need to shed light on the underlying mechanistic pathways and explore novel therapeutic strategies. This study aimed to examine the potential therapeutic effects of Citrus clementine essential oil (CCEO) in treating potassium dichromate (PDC)-induced ALI. The chemical profile of CCEO was created through GC-MS analysis. An in vivo study in rats was conducted to evaluate the effect of CCEO administrated via two different delivery systems (oral/inhalation) in mitigating acute lung injury (ALI) induced by intranasal instillation of PDC. Eight volatile compounds were identified, with monoterpene hydrocarbons accounting for 97.03% of the identified constituents, including 88.84% of D-limonene. CCEO at doses of 100 and 200 mg/kg bw exhibited antioxidant and anti-inflammatory properties. These significant antioxidant properties were revealed through the reduction of malondialdehyde (MDA) and the restoration of reduced glutathione (GSH). In addition, inflammation reduction was observed by decreasing levels of cytokines tumor necrosis factor-α and tumor growth factor-β (TNF-α and TGF-β), along with an increase in phosphatidylinositide-3-kinase (PI3K) and Akt overexpression in lung tissue homogenate, in both oral and inhalation routes, compared to the PDC-induced group. These results were supported by histopathological studies and immunohistochemical assessment of TGF-β levels in lung tissues. These findings revealed that CCEO plays an integral role in relieving ALI induced by intranasal PDC and suggests it as a promising remedy.

Human health risk assessment of metals in soil samples of a Brazilian city with a historic contamination complex.

Rio Grande is a city located on a narrow industrialized and urbanized Brazilian peninsula, characterized by wetlands. Due to population growth, numerous urban backfilled regions were built to expand the territorial area of the city. Currently, more than 60% of the central area of the city comes from the grounding of wetlands. The material used for the expansion of the territory had a history of contamination from metals from the tannery and textile industries (mainly Hg) and urban solid waste. In addition to past sources, the city has an active industrial complex with fertilizer, petrochemical, and grain industries. This study evaluated the risks to human health caused by metals (Hg, Fe, Ni, Cr, Cu, Pb, and Zn) in original soils and backfills, considering the oral, inhalation, and dermal routes of exposure for children and adults using the tool human health risk assessment (HHRA) proposed methodology by USEPA. A total of 63.81% of the original soil samples and 57.14% of the backfill soil samples showed a non-carcinogenic risk (HInc>1) for at least one evaluated metal. Still, approximately 10% of the samples presented carcinogenic risk when the Cr was considered in the hexavalent form. The dermal (Hg, Ni, and Cr) and oral (Fe, Cu, and Zn) exposure routes had the greatest contribution to the total risk. The non-carcinogenic risk for Hg, Cr(VI), and Pb was heterogeneously distributed between the original soils and backfills and associated with the proximity to some pollution sources. Given the complexity of historical occupation in the municipality and the increasing industrialization, both the original areas and the backfills should be included in the risk management strategy to minimize risks.

Human health risk assessment model associated with PM2.5 bound metals in paradip port township, India

This study aimed to assess the environmental risk and human health risks associated with PM2.5-bound metals in Paradip city between January 2019 and December 2021. The seasonal average concentrations of PM2.5 were measured 91.43 ± 70.18 μg m−3, 103.40 ± 60.80 μg m−3, 124.74 ± 62.37 μg m−3, and 159.37 ± 77.88 μg m−3 in pre-monsoon, monsoon, post-monsoon, and winter season respectively. The highest and lowest concentrations are estimated in the winter and pre-monsoon season. Paradip city experienced tropical weather conditions with a hot and humid climate. The wind pattern shows that the predominant wind direction was observed from the south-south-west (SSW) direction. The metals in PM2.5 were analysed using an atomic absorption spectrophotometer (AAS) by air-acetylene flame using a hollow cathode lamp. The average metal concentration decreased in the order of Fe > Al > Zn > Pb > Cr > Mn > Ni > Cu > Co > Cd > As. The value of the geo-accumulation index (Igeo) was evaluated >1 for Cd, Fe, and Zn elements. The health risk assessment (HRA) results showed that non-carcinogenic risk (NCR) was higher through the inhalation route followed by ingestion and dermal contact. The cumulative NCR, which is expressed in terms of the hazard index (HI), is greater than 1 for infant (2.78E+00), child (2.53E+00), and adult (1.04E+00) via inhalation pathway. The total carcinogenic risk (TCR) for infants, children, and adults was estimated at 1.45E-04, 7.24E-05 and 1.25E-05, respectively, which exceeded the acceptable limit of 1.00E-06. Our comprehensive research plays an important role in both policymakers and relevant stakeholders for the preparation of city action plans concerning ambient air pollution, which can improve the air quality in and around Paradip city, India.

Prescription pattern of anti-asthmatic drugs for adults in asthma outpatient department in a tertiary care hospital

Background: Various groups of drugs are prescribed for the management of bronchial asthma. Choosing correct group is necessary for compliance and good outcome. Aims and Objectives: The aims and objectives of the study are to know the prescribing pattern of anti-asthmatic drugs for adults. Materials and Methods: 500 asthma patients prescription were analyzed from the MMC/RGGGH, Chennai. Results: 654 prescriptions were screened and 500 prescriptions were taken for data analysis. The data showed 67% men and 33% women. Most of them received combination therapy (85%) than monotherapy (15%). Beta 2 agonists (salbutamol) formed major anti-asthmatic group (68%). Steroids group formed next (47%). Deriphyllin was prescribed for 108 patients (22%), cetrizine (anti-histamine) for 55 patients (11%), and montelukast (leukotriene antagonists) for 45 patients (9%). About 76% patients took oral tablet and 24% took inhalers. Conclusion: β 2 agonists were preferred more than steroids group. Combination therapy is given more than monotherapy. Majority patients in our study were given oral route when compared to inhalational route.

Удосконалення методології досліджень та оцінки інгаляційного та дермального впливу хімічних засобів захисту рослин. Частина ІІ.

Introduction. In the first part of the article, a comparative analysis of the domestic and international research methodology and assessment of the impact of plant protection products (PPP) on agricultural workers and the population is carried out. The analysis showed that the existing methodological approaches in Ukraine need to be improved. In particular, methodological approaches and procedures for their implementation should comply with international requirements for measuring external inhalation and dermal doses, calculating absorbed doses of the active substance (AS) PPP that affect persons subject to protection. Purpose of the Study. To develop procedures for studying and calculating the impact of the PPP on the operator, worker, outsider and resident during and after the application of the PPP that meet international requirements. Materials and Methods. A scientific analysis of domestic and foreign normative, methodological documents and scientific sources regulating the organization of research, measurement and calculation of the impact of PPP on agricultural workers and the population during and after the use of these products is carried out. Results. Based on the results of the analysis, procedures for sampling, delivery, storage of samples, description of data, calculation of exposure and absorbed doses of AS affecting the body of the operator, worker, outsider and resident by inhalation and dermal routes are proposed. Conclusions. The use of the proposed procedures will ensure that the studies comply with international requirements, and the results of their implementation will ensure the subsequent assessment and management of the PPP exposure risk to protected people. Keywords: plant protection products (PPP), inhalation and dermal exposure, operator, worker, outsider, resident, exposure and absorbed doses.

A control banding method for chemical risk assessment in occupational settings in France.

This study describes a method whose aim is to help companies assess the chemical occupational risks related to labeled products and industrial chemical emissions. The method is intended to be used by industrial hygienists at the scale of one company. Both inhalation and cutaneous exposure routes are taken into account. The method relies on a control-banding scheme. A work situation is described by exposure parameters such as the process or the local exhaust ventilation and by the hazard of the product. Each possible value of the parameters is associated with a "band," which is associated with an integer value. The multiplication of these values results in a score, which represents a priority for intervention. The higher the score, the more the situation warrants investigation for implementing prevention measures, such as chemical substitution and the addition of local exhaust ventilation. To simplify communication, the priority is associated with a colored priority band: red for "very high priority," orange for "high priority," and green for "moderate priority." The priority bands are computed for all work situations performed in a company. An example of the use of this method is described in a French façade insulation company. A tool named Seirich was developed to implement this method and promote good practices for helping industrial hygienists in the prioritization of interventions for reducing chemical risk in France.